The clinical diagnostic industry is on the border of a recombinant protein revolution. Recombinants have been obtainable for decades and have served certain sectors of diagnostics quite well, but they have not been extensively adopted by the industry.
In certain areas, there has been a reluctance to use recombinants because, historically, native proteins performed well and were obtainable in ample supply. Recent declines in performance and availability however will significantly impact the diagnostic industry and stimulate wide support for a move to recombinant protein.
Challenges for Recombinant Proteins
Recombinant proteins have performed well in certain areas of diagnostics in which native proteins are not ample enough in tissues and fluid to support the wide purification demand of the diagnostics industry. In many areas of clinical diagnostics, there has been a reluctance to adopt recombinant protein. The main complaint is that the recombinants obtained in the past didn’t perform as well as native proteins in clinical immunoassays.
These contain areas in which the structure of the aim protein is complex and hard to produce in recombinant form. Some difficult proteins to produce are those that include multiple subunits and those that are glycosylated or undergo some other post-translational modifications.
Novel Recombinant Proteins
In the zone of reproductive biology, recombinant forms of HCG (human chorionic gonadotrophin) FSH (follicle-stimulating hormone), and LH ( luteinizing hormones have been obtainable. Structurally all these three hormones are heterodimer glycoproteins, creating them difficult recombinant targets. Recombinant forms of hCG, FSH, and LH have not been used in the diagnostic industry.
Researchers have found one of the most complicated proteins in clinical diagnosis is ferritin an iron storage protein composed of heavy and light chain subunits. The form of heavy and light chains varies, and many forms of ferritin are known to exist, which presents an additional difficulty to recombinant protein constructors. recombinant ferritin has been available for more than 29 years, but because of these structural complexities, it has not been followed by the diagnostic industry until now.
Clinical Diagnostic Industry Benefits from Recombinant Proteins
Multiplex recombinant proteins can be produced to perform comparably to their native counterparts. If performance is better the 2 is homologous, recombinant proteins are the selected option in the clinical diagnostic industry, as they offer several benefits over native proteins.
- Readily Available at Reasonable Prices
It is one of the primary benefits of recombinant protein and the sustainable nature of their starting material. Recombinants are sourced from cell lines that are grown and harvested on demand, which makes the purified protein accessible when required. Native tissue procurement is unpredictable as it is dependent on the obtainability of single donors.
- Nil Interference From Protein Contaminants
Several native proteins are purified from initiate material that includes proteins that are similar to the aimed protein being purified. These contaminating protein are troublesome because they may cause intervention in immunoassays and contributes to a long purification cycle.
- No Disease Testing
Native humans protein are purified from materials that run the risk of caring infection diseases agents like protein purified from native sources can present regulatory challenges for diagnostic test kit manufacturers. Recombinant proteins are purified from cell culture systems that are not human-sourced and are not bio-hazardous.
GeNext Genomics has precise expertise in the industry of recombinant protein production service for decades now and strives to deliver high-quality biologics with the recombinant protein service. While there is huge industry demand for biologics and services, we have a team of expert scientists and trained professionals, who are driven by science to make life science customers get one-stop solutions at GeNext Genomics.
We have expertise in protein purification and expression of recombinant proteins using bacterial, animal, and plant vectors, yet there are many discoveries ahead which keep us going.